Introduction
 
Acetone sterilisation with 100% anhydric acetone is an excellent alternative to autoclaving, chemical sterilisation or gas sterilisation of instruments. The action of acetone is based on the biochemical disruption of the lipid membranes of pathogenic organisms.

The basic approach to acetone sterilisation demands cleaning by washing with water and soap and careful drying of the instruments. Any remaining pathogenetic organisms will be killed by acetone 100%.

A disadvantage of acetone is its toxicity wherefore it shall be kept in a special tightly closed box with a safety lock and a special silicone sealing plate.
The acetone may be left in the box on condition that no wet instruments are placed in it.

Acetone is volatile but not explosive. Direct fire to the acetone will light up the acetone, in which case it becomes a serious fire hazard.